My second encounter with TCC-3.3ICE-N was much better than my first. It is partially due to GFP’s higher light intensity. I was able to take my first shot in 1o minutes after setup. There are five different settings for focus mode at higher gain and shorter exposure. 2.5 or 5 fps seems to be more acceptable for setting up focus. After I obtained satisfactory preview image in focus mode I switched to fine mode at lower gain (12db) and longer exposure time (approx 5 sec) to get cleaner picture.
Photos of Green Fluorescent Protein (GFP) Expression in K562 cell line – photographed with Tucsen TCC-3.3ICE-N microscope camera
April 1, 2010 by Labchef
My previous impression with Tucsen TCC-3.3ICE-N was mixed. I am very positive about the picture quality of TCC-3.3ICE-N but I am not too thrill about the software. Today, I was hoping to use MICAM to take fluorescent photos. I have done several shot with Tucsen CMOS model with MICAM. MICAM seems to do a better job in adjusting auto exposure and white balance. Unfortunately, TCC-3.3ICE-N does not come with a DirectShow driver like the CMOS model so I ended up still using TSView 220.127.116.11.
Tucsen TCC-3.3ICE-N microscope was mounted to the side of Zeiss Axiovert M200 inverted microscope. The green fluorescent protein (GFP) is excited under blud ligh and fluoresce green.