Guppies (Poecilia reticulata) is the most popular aquarium fish. They are community fish which means that they get alone with other aquarium fish. They are not very picky about water condition and easy to breed. I have a tank of guppies that I have kept for several generations.
Fish fins are transparent or semi-transparent. They can be easily observed under microscope without any sample preparation. I took five guppies from my fish tank and put them in a small container next to my microscope so I can easily put the fish back in the water (They can’t be leave out of water for extended period of time). I lay the fish on a slide then put a piece of moistened cotton ball on the head. It servers two purpose: 1. Put some weight on the fish so it wouldn’t jump. 2. Prevent it from drying out too quickly (The fish won’t be able to breathe but I think a little bit moisture will help). I tried to put the cover slide on but they were quickly move by fish’s powerful tail. I end up not using the cover slide. For this reason, the observation is only limited to 40X and 100X. The working distance of 40X objective is too short.
The blood circulation is recorded at 100X with Tucsen Microscope Camera.